VOI - Voice of Indonesia | RRI - Radio Republik Indonesia

Treatment and prevention of acute wounds, such as trauma wounds, sutures, and abrasions, is important so that they do not turn into chronic wounds. Wounds must be treated to avoid mild to severe complications. When a wound is formed, complications can be triggered by the presence of bacteria entering, causing infection. Therefore, treatment must be carried out effectively to avoid ongoing infection. Wound treatment generally uses plaster. However, commercial plasters currently circulating mostly do not contain active ingredients so they are less effective in wound healing. The use of plaster as a wound cover can also interfere with the aesthetics of the skin if it is used for a long time. Therefore, to get an acute wound healing drug that is effective and can be used comfortably, a group of Gadjah Mada University students, namely Aufa Lufhf Ambar Verisandri, Bondan Setyoko, Galih Patria, Ni Luh Wayan Putri Dewi Angelina and Alma Rizki Fadila conducted a study on nano-spray with a combination of fragrant Pandan leaf extract and clove flower as an anti-inflammatory, antiseptic, and topical anesthetic for healing acute skin wounds.

Aufa, one of the students, said that in this study, crab shell chitosan was used in combination nano-spray. This material is easy to absorb and can form a film membrane that can act as a carrier for the active drug ingredient. To make nano-spray, it is done by extracting fragrant Pandan leaves and clove flowers. Furthermore, both extracts are combined and other ingredients are added to make an emulsion preparation first. This emulsion preparation is then processed until homogeneous and produces nano-sized particles. The nano-mulsion that has been formed is then put into a spray bottle so that it becomes a nano-spray. Meanwhile, Bondan and friends added that in their research, they conducted a number of tests on nano-spray preparations. Several tests were carried out, namely organoleptic test, specific gravity test, nanoparticle size test, pH test, viscosity test, stability test, molecular docking, and minimum bacterial inhibition level test. The measurement results using the Particle Size Analyzer show that the average diameter of the synthesized nano-particles is 66.36 nano-meters. It has met the requirements for nano-emulsion dosage sizes, which are in the range of 10 to 1000 nano-meters.

Bondan further explained that the nano-spray had an average pH of 4.33. The pH value is in accordance with the pH of the skin and will not pose a danger in its use. In addition, for other quality tests such as viscosity level and specific gravity, the formed nano-spray has been tested with good quality. Then, the stability test of the preparation showed that the nano-spray preparation remained stable in its shape both at low and high temperatures. Next, in terms of quality, the results of the test on this preparation showed that this combination nano-spray already has good quality, is easy to use, and not easily damaged. Furthermore, from the minimum inhibitory bacteria test, it was shown that the inhibition zone caused by the nano-spray combination of Pandan fragrant leaf extract and clove flower extract was significantly larger than the red wound medicine. Thus, it can be concluded that the Pandan Wangi leaf extract nano-spray and clove flower have a better antiseptic effect than red wound medicine. Anti-inflammatory and anesthetic effects were carried out in silico molecular docking to see the strength of the bond between the active ingredient and human receptors related to anti-inflammatory and anesthetic effects. The molecular docking results showed that the nano-spray had a stronger bond than the active ingredient of the red drug on the human receptor. This proves that this combination nano-spray has a better anti-inflammatory and anesthetic effect than red sore medicine.